Highly motivated Project Associate and PhD Candidate with a profound interest in Animal Pathology, Experimental/In Vivo Pharmacology, Molecular-Cellular Biology, and advanced Analytical Instrumentations. Committed to driving significant impacts in developing therapeutic solutions and enhancing patient outcomes through rigorous pre-clinical studies and novel formulation development. Adept at leveraging advanced research methodologies and collaborating effectively within multicultural, multidisciplinary teams.
Project Associate, PhD Candidate (Full-time)
Solan, Himachal Pradesh, India
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Summary
As Project Associate and PhD Candidate, I am currently leading the development of a novel synbiotic herbal formulation to mitigate obesity-induced metabolic and immune dysfunctions under an ICMR project.
Highlights
Orchestrated comprehensive microbiology experiments, including broth preparation, inoculation, serial dilution, streak plating, and Colony Forming Unit (CFU) quantification.
Developed and executed *in vitro* senescence models using C2C12 and RAW 264.7 cell lines, performing Senescence β-Galactosidase Staining, Phagocytosis Assay, and EVOS FL cell imaging.
Established and applied *in vitro* hyperlipidemia-hyperglycemia models utilizing 3T3-L1 and Hep G2 cell lines for targeted therapeutic investigation.
Project Associate-1 (Full-time)
Palampur, Himachal Pradesh, India
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Summary
As Project Associate-1, I led the design and execution of pre-clinical studies, successfully developing immunomodulatory nutraceutical formulations for lung inflammation and allergic asthma.
Highlights
Designed and executed comprehensive experimental plans (DoE) for pre-clinical studies, managing resources to successfully develop nutraceutical formulations targeting lung inflammation and allergic asthma.
Conducted *in vivo* and *in vitro* model studies using Ovalbumin (OVA) and Lipopolysaccharides (LPS), validating efficacy on murine models (Balb/c).
Performed cytotoxicity studies via ROS-NO-MTT assays in RAW 264.7 Macrophages, ensuring formulation safety and efficacy.
Evidenced formulation efficacy through diverse approaches including ELISA, Western blots, qRT-PCR, Histopathology (H&E Staining), and solubility/stability studies.
Mentored and assisted 2 MD(BAMS)-MSc thesis trainees in lab experiments and animal models, fostering team development.
Research Assistant (Full-time)
New Delhi, Delhi, India
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Summary
As a Research Assistant, I managed patient data collection and operations for the Human Microbiome Initiative project, successfully leading a team of 4 in comprehensive project management activities, ensuring efficient execution of the project.
Highlights
Coordinated patient sample collection and nutritional data gathering, utilizing Epicollect5 software for robust data management.
Laboratory Technician (Full-time)
New Delhi, Delhi, India
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Summary
As a Laboratory Technician, I performed routine laboratory work in the Serology section, efficiently handling patient sample collection and processing.
Highlights
Executed routine laboratory procedures within the Serology section, ensuring accurate patient sample collection and processing.
Managed follow-up lab work, contributing to efficient workflow and timely results delivery.
Master Thesis Researcher
New Delhi, Delhi, India
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Summary
As a Master Thesis Researcher, I assessed the *in vitro* DNA-Protein radioprotective activity of novel drugs, contributing to pre-clinical formulation efficacy evaluation.
Highlights
Assessed the efficacy of novel formulations in *in vitro* and *in vivo* models (C57BL/6 Mice) for DNA-Protein radioprotection.
Evaluated pre-clinical formulation efficacy using Agarose gel electrophoresis, SDS-PAGE, and Comet assay techniques.
Utilized COBALT-60 Radio-Isotope Radiation as a primary source for experimental studies.
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M.Sc.
Microbiology
Grade: 1st Division
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B.Sc.
Chemistry, Microbiology, Botany
Grade: 2nd Division
Published by
Peptides
Summary
Research on how Leupeptin maintains redox homeostasis by targeting the ROS-autophagy-inflammatory axis in LPS-stimulated macrophages and Con-A challenged lymphocytes.
Western Blot, ELISA, qRT-PCR, Agarose Gel Electrophoresis, SDS-PAGE, DNA-RNA-Protein Extraction, NANODROP, Bradford Assay.
Cell Culture (C2C12, RAW 264.7, 3T3-L1, HepG2), Primary Cell Culture (spleen, peritoneal macrophages), Comet Assay, ROS Assay, NO Assay, MTT Assay, Phagocytosis Assay, EVOS FL Cell Imaging.
Tissue Processing (Fixation, Embedding, Sectioning), Staining (H&E, Toluidine Blue).
C57BL/6 Mice, BALB/c Mice, Anesthesia, Euthanasia, Necropsy, Drug Oral Dosing (Intratracheal, Intraperitoneal, Intramuscular, Intradermal), Retro-orbital Puncture, Blood Collection, Peritoneal Macrophage Isolation, Phlebotomy, Human IV-IM Injections.
DPPH Assay, TPC Assay, TFC Assay, ABTS Assay, Nutraceutical Formulation (Powder-Syrup, in vitro Creams).
Broth Preparation, Bacterial Isolation-Culture, Inoculation, Serial Dilution, Streak Plating Method, Colony Forming Units (CFUs), Gram Staining, Light Microscopy.
MS Office Suite, Origin 8.5, GraphPad Prism 9, ImageJ, ChemDRAW, CorelDRAW Suite, Epicollect5.
Experimental Design (DoE), Pre-clinical Studies, In vitro Models, In vivo Models, Good Laboratory Practices (GLP), Good Clinical Practices (GCP).
Patient Sample Collection, Report Writing, SOP Development, Purchase Indents, Lab Management.
Project Management, Mentorship, Cross-functional Collaboration.
